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. 2019 Jun 4;6(2):64.
doi: 10.3390/medicines6020064.

Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves

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Free PMC article

Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves

Tran Duc Viet et al. Medicines (Basel). .
Free PMC article

Abstract

Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50-70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.

Keywords: Celastrus hindsii; ESI-MS; GC-MS; antioxidant activity; total flavonoids; total phenolics.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Fractionation of EtOAc extract from powder of C. hindsii leaves.
Figure 2
Figure 2
The relationship between antioxidant activity and total phenolic contents of C. hindsii by 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging method.
Figure 3
Figure 3
The relationship between antioxidant activities and total phenolic of C. hindsii by ABTS radical scavenging activity method.

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