A Unique Triculture Model to Study Osteoblasts, Osteoclasts, and Endothelial Cells

Tissue Eng Part C Methods. 2019 Jul;25(7):421-432. doi: 10.1089/ten.TEC.2018.0301.


In this article, we first developed a new medium to culture together primary human osteoblastic, osteoclastic, and endothelial cells (ECs) chosen to represent the three major bone cell tissues. Indeed, no study has been conducted on primary human cells and on the phenotype/activity retention of these three primary human cell types. Thus, we established an original triculture model with osteoblastic, osteoclastic, and ECs, where not only both cell phenotype and cell activity were maintained but also cell culture homeostasis. These promising results will permit further investigations to create in vitro conditions to mimic the bone microenvironment and analyze cell interactions in ex vivo studies.

Keywords: cell phenotype; culture medium; human primary cells; triculture model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Coculture Techniques
  • Culture Media / pharmacology
  • Endothelial Cells / cytology*
  • Endothelial Cells / drug effects
  • Humans
  • Models, Biological*
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • Osteoclasts / cytology*
  • Osteoclasts / drug effects
  • Osteogenesis / drug effects
  • Phenotype
  • Tartrate-Resistant Acid Phosphatase / metabolism


  • Culture Media
  • Tartrate-Resistant Acid Phosphatase