Purpose of review: The recent emergence of single-cell technologies has permitted unprecedented insight into the molecular drivers of fate choice in blood stem and progenitor cells. This review gives a broad overview of current efforts to understand the molecular regulators of malignant hematopoietic stem cells (HSCs) at the single-cell level.
Recent findings: The large-scale adoption of single-cell approaches has allowed extensive description of the transcriptional profiles and functional properties of single HSCs. These techniques are now beginning to be applied to malignant HSCs isolated directly from patients or from mouse models of malignancy. However, these studies have generally struggled to pinpoint the functional regulators of malignant characteristics, since malignant HSCs often differ in more than one property when compared with normal HSCs. Moreover, both normal and malignant populations are complicated by HSC heterogeneity.
Summary: Despite the existence of single-cell gene expression profiling tools, relatively few publications have emerged. Here, we review these studies from recent years with a specific focus on those undertaking single-cell measurements in malignant stem and progenitor cells. We anticipate this to be the tip of the iceberg, expecting the next 2-3 years to produce datasets that will facilitate a much broader understanding of malignant HSCs.
Differentiation-based model of hematopoietic stem cell functions and lineage pathways.Blood. 2018 Sep 13;132(11):1106-1113. doi: 10.1182/blood-2018-03-791517. Epub 2018 Jul 24. Blood. 2018. PMID: 30042097 Free PMC article. Review.
Machine learning predicts putative hematopoietic stem cells within large single-cell transcriptomics data sets.Exp Hematol. 2019 Oct;78:11-20. doi: 10.1016/j.exphem.2019.08.009. Epub 2019 Sep 9. Exp Hematol. 2019. PMID: 31513832 Free PMC article.
The biochemistry of hematopoietic stem cell development.Biochim Biophys Acta. 2013 Feb;1830(2):2395-403. doi: 10.1016/j.bbagen.2012.10.004. Epub 2012 Oct 12. Biochim Biophys Acta. 2013. PMID: 23069720 Free PMC article. Review.
Single-cell approaches identify the molecular network driving malignant hematopoietic stem cell self-renewal.Blood. 2018 Aug 23;132(8):791-803. doi: 10.1182/blood-2017-12-821066. Epub 2018 Jul 10. Blood. 2018. PMID: 29991556 Free PMC article.
Molecular profiling of candidate human hematopoietic stem cells derived from human embryonic stem cells.Exp Hematol. 2008 Nov;36(11):1436-48. doi: 10.1016/j.exphem.2008.06.001. Epub 2008 Aug 9. Exp Hematol. 2008. PMID: 18694618
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Impact of mesenchymal stem cell senescence on inflammaging.BMB Rep. 2020 Feb;53(2):65-73. doi: 10.5483/BMBRep.2020.53.2.291. BMB Rep. 2020. PMID: 31964472 Free PMC article.