Cooperative 2'-O-methylation of the wobble cytidine of human elongator tRNA Met(CAT) by a nucleolar and a Cajal body-specific box C/D RNP

Genes Dev. 2019 Jul 1;33(13-14):741-746. doi: 10.1101/gad.326363.119. Epub 2019 Jun 6.

Abstract

Site-specific 2'-O-ribose methylation of mammalian rRNAs and RNA polymerase II-synthesized spliceosomal small nuclear RNAs (snRNAs) is mediated by small nucleolar and small Cajal body (CB)-specific box C/D ribonucleoprotein particles (RNPs) in the nucleolus and the nucleoplasmic CBs, respectively. Here, we demonstrate that 2'-O-methylation of the C34 wobble cytidine of human elongator tRNAMet(CAT) is achieved by collaboration of a nucleolar and a CB-specific box C/D RNP carrying the SNORD97 and SCARNA97 box C/D 2'-O-methylation guide RNAs. Methylation of C34 prevents site-specific cleavage of tRNAMet(CAT) by the stress-induced endoribonuclease angiogenin, implicating box C/D guide RNPs in controlling stress-responsive production of putative regulatory tRNA fragments.

Keywords: 2′-O-methylation; angiogenin; box C/D small nucleolar RNA; small Cajal body RNA; tRNA-derived fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Nucleolus / metabolism*
  • Coiled Bodies / metabolism*
  • Cytidine / metabolism*
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Methylation
  • RNA, Small Nucleolar / genetics
  • RNA, Small Nucleolar / metabolism
  • RNA, Transfer / genetics
  • RNA, Transfer / metabolism*
  • Ribonuclease, Pancreatic / metabolism
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism*
  • Stress, Physiological

Substances

  • RNA, Small Nucleolar
  • Ribonucleoproteins
  • Cytidine
  • RNA, Transfer
  • angiogenin
  • Ribonuclease, Pancreatic