Design Approaches for Generating Organ Constructs

Cell Stem Cell. 2019 Jun 6;24(6):877-894. doi: 10.1016/j.stem.2019.05.016.

Abstract

Organ constructs are organ-like structures grown in vitro or in vivo that harbor the components, architecture, and function of in vivo organs, in part or in toto. The convergence of stem cell biology, bioengineering, and gene editing tools have substantially broadened our ability to generate various types of organ constructs for regenerative medicine as well as to address pressing biomedical questions. In this Review, we highlight prevailing approaches for generating organ constructs, from organoids to chimeric organ engineering. We also discuss design principles of different approaches, their utility and limitations, and propose strategies to resolve existing hurdles.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cellular Reprogramming
  • Gene Editing
  • Humans
  • Induced Pluripotent Stem Cells / pathology
  • Induced Pluripotent Stem Cells / physiology*
  • Organogenesis
  • Organoids / pathology*
  • Organoids / physiology
  • Regenerative Medicine
  • Tissue Banks
  • Tissue Engineering / methods*