Altered levels of placental miR-338-3p and miR-518b are associated with acute chorioamnionitis and IL6 genotype

Chaini Konwar; Irina Manokhina; Jefferson Terry; Amy M Inkster; Wendy P Robinson

doi:10.1016/j.placenta.2019.05.009

Altered levels of placental miR-338-3p and miR-518b are associated with acute chorioamnionitis and IL6 genotype

Placenta. 2019 Jul:82:42-45. doi: 10.1016/j.placenta.2019.05.009. Epub 2019 May 23.

Authors

Chaini Konwar¹, Irina Manokhina², Jefferson Terry³, Amy M Inkster⁴, Wendy P Robinson⁵

Affiliations

¹ BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada. Electronic address: chaini@alumni.ubc.ca.
² BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada. Electronic address: imanokhina@uvic.ca.
³ BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Department of Pathology, BC Children's Hospital, BC V6H 3N1, Canada. Electronic address: jefferson.terry@cw.bc.ca.
⁴ BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada. Electronic address: ainkster@bcchr.ca.
⁵ BC Children's Hospital Research Institute, Vancouver, BC V5Z 4H4, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC V6H 3N1, Canada. Electronic address: wrobinson@bcchr.ca.

PMID: 31174625
DOI: 10.1016/j.placenta.2019.05.009

Abstract

Placental-derived miRNAs are attractive candidates as biomarkers of placental health, but their associations with specific pathologies, such as acute chorioamnionitis (aCA), are not well explored. Samples of chorionic villi from 57 placentas (33 aCA and 24 non-aCA) were analyzed. Expression was quantified for six candidate miRNAs (miR-146a, miR-210, miR-223, miR-338-3p, miR-411, and miR-518b), using quantitative real-time PCR. miR-518b and miR-338-3p were differentially expressed between aCA cases and non-aCA cases (Bonferroni-corrected p < 0.05). Further, we observed that placental miR-518b expression was associated with an IL6 SNP (rs1800796), a polymorphism we previously reported as a risk-conferring variant for aCA.

Keywords: Acute chorioamnionitis; Biomarkers; Placenta; Preterm birth; miRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Chorioamnionitis / genetics
Chorioamnionitis / metabolism*
Chorionic Villi / metabolism
Female
Gene Expression Regulation
Genotype
Humans
Interleukin-6 / genetics*
MicroRNAs / genetics
MicroRNAs / metabolism*
Placenta / metabolism*
Polymorphism, Single Nucleotide*
Pregnancy
Young Adult

Substances

Interleukin-6
MIRN338 microRNA, human
MIRN518 microRNA, human
MicroRNAs

Grants and funding

49520 /CIHR/Canada