Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies

Mayo Clin Proc. 1987 Nov;62(11):969-77. doi: 10.1016/s0025-6196(12)65066-6.


We measured plasma cell labeling indices (LI) in 52 patients with monoclonal gammopathies. Cytoplasmic reactivity with polyspecific or kappa- and lambda-specific light chain anti-Ig reagents identified monoclonal plasma cells, plasmablasts, and lymphocytoid plasma cells. Among newly diagnosed untreated patients, a high immunofluorescence LI distinguished those with multiple myeloma (MM) from those with stable monoclonal gammopathies (P less than 0.002). Among treated patients with MM, those in the plateau phase of the disease had low LI, whereas patients in the relapse phase or early in treatment had high LI. The immunofluorescence LI correctly classified three more patients with newly diagnosed MM than did the tritiated thymidine LI technique. LI specific for the neoplastic plasma cells resulted in excellent discrimination of patients with active disease. Because results are easily and rapidly obtained, this technique is useful clinically.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloidosis / diagnosis
  • Amyloidosis / immunology
  • Antibodies, Monoclonal / immunology
  • Autoradiography
  • Cytoplasm / immunology
  • Diagnosis, Differential
  • Evaluation Studies as Topic
  • Fluorescent Antibody Technique*
  • Humans
  • Immunoglobulin Light Chains / immunology
  • Immunoglobulin gamma-Chains / immunology
  • Leukemia, Plasma Cell / diagnosis*
  • Leukemia, Plasma Cell / immunology
  • Multiple Myeloma / classification
  • Multiple Myeloma / diagnosis*
  • Multiple Myeloma / immunology
  • Paraproteinemias / diagnosis*
  • Paraproteinemias / immunology
  • Plasma Cells / analysis
  • Plasma Cells / immunology
  • Prognosis


  • Antibodies, Monoclonal
  • Immunoglobulin Light Chains
  • Immunoglobulin gamma-Chains