Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis

Int Immunopharmacol. 2019 Sep;74:105676. doi: 10.1016/j.intimp.2019.105676. Epub 2019 Jun 7.

Abstract

In this study, the anti-inflammatory mechanisms of Quercetin (Que) on atopic dermatitis (AD)-like skin lesions was examined. The left ear of mice was applied with MC903, followed by Que. administration daily on the ear for 8 days. Then macroscopic and histologic examination was performed to detect the severity of skin lesions. In the skin section of AD mice, we observed that Que. could reduce the expression of CCL17, CCL22, IL-4, IL-6, IFN-γ and TNF-α. In vitro, the anti-inflammatory effects of Que. were examined on human keratinocytes (HaCaT cells) treated with IFN-γ/TNF-α. To unveil the lncRNAs' regulatory role on Que-activated anti-inflammatory function, the next-generation high-throughput sequencing was performed in HaCat cells with or without Que. treatment, which profiled the expression of lncRNAs and mRNAs, the results illustrated that lnc-C7orf30-2, a lncRNA expressed differentially, was correlated with IL-6 expression. Silencing of lnc-C7orf30-2 by RiboTM lncRNA Smart Silencer proved its role on IL-6 expression. Therefore, the results here demonstrated that topical administration of Que. plays a beneficial role in controlling AD symptoms, which may serve as potential candidate for AD treatment.

Keywords: Anti-inflammatory; Atopic dermatitis; Quercetin.

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Calcitriol / analogs & derivatives
  • Cell Line
  • Cell Survival / drug effects
  • Cytokines / immunology
  • Dermatitis, Atopic / chemically induced
  • Dermatitis, Atopic / drug therapy*
  • Dermatitis, Atopic / immunology
  • Dermatitis, Atopic / pathology
  • Disease Models, Animal
  • Female
  • Humans
  • Mice, Inbred C57BL
  • Quercetin / pharmacology
  • Quercetin / therapeutic use*
  • Skin / drug effects
  • Skin / immunology
  • Skin / pathology

Substances

  • Anti-Inflammatory Agents
  • Cytokines
  • calcipotriene
  • Quercetin
  • Calcitriol