Peppermint Essential Oil-Doped Hydroxyapatite Nanoparticles With Antimicrobial Properties

Molecules. 2019 Jun 9;24(11):2169. doi: 10.3390/molecules24112169.


This study aimed at developing an antimicrobial material based on hydroxyapatite (HAp) and peppermint essential oil (P-EO) in order to stimulate the antimicrobial activity of hydroxyapatite. The molecular spectral features and morphology of the P-EO, HAp and hydroxyapatite coated with peppermint essential oil (HAp-P) were analyzed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The coating of the HAp with the P-EO did not affect the ellipsoidal shape of the nanoparticles. The overlapping of IR bands of P-EO and HAp in the HAp-P spectrum determined the formation of the broad molecular bands that were observed in the spectral regions of 400-1000 cm-1 and 1000-1200 cm-1. The antibacterial activity of the P-EO, HAp and HAp-P were also tested against different Gram-positive bacteria (methicillin-resistant Staphylococcus aureus (MRSA) 388, S. aureus ATCC 25923, S. aureus ATCC 6538, E. faecium DSM 13590), Gram-negative bacteria (Escherichia coli ATCC 25922, E. coli C5, P. aeruginosa ATCC 27853, P. aeruginosa ATCC 9027) and a fungal strain of Candida parapsilosis. The results of the present study revealed that the antimicrobial activity of HAp-P increased significantly over that of HAp.

Keywords: antimicrobial properties; hydroxyapatite; peppermint essential oil.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacteria / drug effects
  • Durapatite / chemistry*
  • Fungi / drug effects
  • Microbial Sensitivity Tests
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Oils, Volatile / pharmacology*
  • Particle Size
  • Plant Oils / pharmacology*
  • X-Ray Diffraction


  • Anti-Bacterial Agents
  • Oils, Volatile
  • Plant Oils
  • Durapatite
  • peppermint oil