In Vitro and in Vivo Studies on the Angiotensin-Converting Enzyme Inhibitory Activity Peptides Isolated from Broccoli Protein Hydrolysate

J Agric Food Chem. 2019 Jun 19;67(24):6757-6764. doi: 10.1021/acs.jafc.9b01137. Epub 2019 Jun 7.


In the present study we purified and identified peptides from broccoli protein hydrolysates and evaluated their angiotensin-converting enzyme (ACE) inhibitory activity in vitro and hypotensive effect in vivo. Three ACE inhibitory peptides were isolated and identified as IPPAYTK, LVLPGELAK, and TFQGPPHGIQVER, and their inhibitory IC50 values were 23.5, 184.0, and 3.4 μM, respectively. We then investigated the effect of gastrointestinal digestion on ACE inhibitory activity. We detected almost two times the ACE inhibitory activity of the peptide LVLPGELAK following simulated digestion than prior to digestion. LVLPGE and LAK, two novel peptides exhibiting high ACE inhibitory activity, were discovered following digestion and possessed IC50 values of 13.5 and 48.0 μM, respectively. The hypotensive effect of the peptides was assessed after oral administration to spontaneous hypertensive rats (SHRs). We found that LVLPGE and LAK demonstrated a significant hypotensive effect in vivo. Protein from broccoli may thus constitute a potential antihypertensive peptide source.

Keywords: ACE inhibitory peptides; broccoli protein; identification; in vivo hypotensive effect; simulated gastrointestinal digestion.

MeSH terms

  • Administration, Oral
  • Angiotensin-Converting Enzyme Inhibitors / administration & dosage*
  • Angiotensin-Converting Enzyme Inhibitors / chemistry
  • Angiotensin-Converting Enzyme Inhibitors / isolation & purification
  • Animals
  • Antihypertensive Agents / administration & dosage*
  • Antihypertensive Agents / chemistry
  • Antihypertensive Agents / isolation & purification
  • Blood Pressure / drug effects
  • Brassica / chemistry*
  • Female
  • Humans
  • Hypertension / drug therapy*
  • Hypertension / enzymology
  • Hypertension / physiopathology
  • Peptide Mapping
  • Peptides / administration & dosage*
  • Peptides / isolation & purification
  • Peptidyl-Dipeptidase A / chemistry
  • Peptidyl-Dipeptidase A / metabolism
  • Plant Proteins / chemistry*
  • Protein Hydrolysates / chemistry
  • Rats
  • Rats, Inbred SHR


  • Angiotensin-Converting Enzyme Inhibitors
  • Antihypertensive Agents
  • Peptides
  • Plant Proteins
  • Protein Hydrolysates
  • Peptidyl-Dipeptidase A