Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide

Miaorong Zhang; Na Yang; Yuxiang Liu; Jianguo Tang

doi:10.1016/j.enzmictec.2019.04.016

Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide

Enzyme Microb Technol. 2019 Sep:128:22-25. doi: 10.1016/j.enzmictec.2019.04.016. Epub 2019 May 3.

Authors

Miaorong Zhang¹, Na Yang², Yuxiang Liu³, Jianguo Tang²

Affiliations

¹ Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, People's Republic of China. Electronic address: mrzhang2018@qdu.edu.cn.
² Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, People's Republic of China.
³ College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, People's Republic of China.

PMID: 31186106
DOI: 10.1016/j.enzmictec.2019.04.016

Abstract

A catalase-inorganic hybrid nanoflowers (Cat-HNFs)-based colorimetric platform was prepared for visual detection of hydrogen peroxide (H₂O₂). Catalase via coordination was self-assembled and embedded in the copper phosphate crystals by sonication. Under the designed colorimetric system, Cat-HNFs showed linear absorbance responses toward H₂O₂ in the concentration range of 1 μM to 1 mM, and the detection limit was 1 μM. Compared to free catalase, Cat-HNFs exhibited enhanced activity and stability for H₂O₂ detection. Moreover, after 5 cycles via centrifugal recovery, Cat-HNFs still remained 68% of their initial activity. The detection result in human serum suggested Cat-HNFs can be a promisng candidate for the detection of H₂O₂ in biomedical field.

Keywords: Colorimetric detection; Hybrid nanoflowers; Hydrogen peroxide; Sonication.

MeSH terms

Biosensing Techniques / methods*
Calorimetry / methods*
Catalase / chemistry
Catalase / metabolism*
Copper / chemistry
Copper / metabolism*
Hydrogen Peroxide / analysis*
Nanostructures / chemistry*
Phosphates / chemistry
Phosphates / metabolism*
Sonication

Substances

Phosphates
Copper
Hydrogen Peroxide
Catalase