Analysis of polyamines as their dabsyl derivatives by reversed-phase high-performance liquid chromatography

Anal Biochem. 1987 Jul;164(1):261-6. doi: 10.1016/0003-2697(87)90395-2.

Abstract

The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadaverine / analysis
  • Chromatography, High Pressure Liquid*
  • Hydrogen-Ion Concentration
  • Polyamines / analysis*
  • Putrescine / analysis
  • Salmonella typhimurium / analysis
  • Spermidine / analysis
  • Spermine / analysis
  • p-Dimethylaminoazobenzene / analogs & derivatives*

Substances

  • Polyamines
  • Spermine
  • 4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride
  • p-Dimethylaminoazobenzene
  • Cadaverine
  • Spermidine
  • Putrescine