[Mechanisms of polyclonal tolerance induced by lipopolysaccharide and cyclophosphamide]

Biull Eksp Biol Med. 1987 Oct;104(10):483-5.
[Article in Russian]


The treatment of recipient mice with LPS from S. marcescens followed by the injection of CY 48 h later inhibited a subsequent antibody production against unrelated antigen (SRBC) and polyclonal mitogen (LPS from Br. abortus). Such a reactivity persisted for 2-3 weeks after treatment. It was shown that the number of Ig+ cells in the spleens of treated mice was decreased, while the population of spleen Thy-1.2+ cells remained unaltered. Cell-cooperative test revealed that the function of B cells, but not T cells, was inhibited by the treatment. There were no changes in DTH response to SRBC. Thus, a subsequent treatment of mice with LPS and CY led to B-cell deficiency. The nature of this phenomenon is presumably the same as the nature of CY-induced antigen-specific immunological tolerance.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibody-Producing Cells / drug effects
  • Antibody-Producing Cells / immunology
  • Brucella abortus*
  • Cyclophosphamide / pharmacology*
  • Cytotoxicity Tests, Immunologic
  • Erythrocytes / immunology
  • Hypersensitivity, Delayed / immunology
  • Immune Tolerance / drug effects*
  • Lipopolysaccharides / pharmacology*
  • Lymphocyte Cooperation / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Serratia marcescens*
  • Time Factors


  • Lipopolysaccharides
  • Cyclophosphamide