Mutations altering the structure of epidermal growth factor-like coding sequences at the Drosophila Notch locus

Cell. 1987 Nov 20;51(4):539-48. doi: 10.1016/0092-8674(87)90123-1.


The Drosophila neurogenic protein Notch is largely composed of tandemly repeated copies of an epidermal growth factor-like sequence. Notch protein contains 36 EGF-like elements, but no two are identical. In the present study, eight mutations are correlated with single amino acid substitutions in EGF-homologous elements of this protein. Genetic analyses of the mutations and comparisons of DNA from mutant and wild-type flies indicate that differentiation of function exists among the tandemly repeated EGF-like sequences and that the total number of repeats and minor variations from the consensus repeat sequence are important for wild-type Notch protein function. One group of EGF-like repeats may be involved in the formation of functional Notch dimers or multimeric proteins. EGF-like elements in a second region of the Notch protein appear to govern its interaction with the product of Enhancer of split, another neurogenic locus of D. melanogaster.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Drosophila melanogaster / genetics*
  • Epidermal Growth Factor / genetics
  • Gene Expression Regulation
  • Genes
  • Genes, Lethal
  • Growth Substances / genetics*
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Recombination, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid


  • Growth Substances
  • Epidermal Growth Factor