Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen

J Proteome Res. 2019 Aug 2;18(8):3052-3066. doi: 10.1021/acs.jproteome.9b00167. Epub 2019 Jun 27.

Abstract

Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree (Olea europaea L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl cis-trans isomerase, cyclophilin, produced in Escherichia coli, was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal, and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food, and animal extracts.

Keywords: allergen; allergenome; cross-reactivity; cyclophilin; in-depth proteomics; olive pollen proteome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics*
  • Allergens / immunology
  • Allergens / isolation & purification
  • Amino Acid Sequence / genetics
  • Animals
  • Antigens, Plant / genetics*
  • Child
  • Chromatography, Liquid
  • Cross Reactions
  • Cyclophilins / genetics*
  • Cyclophilins / immunology*
  • Humans
  • Immunoglobulin E / genetics
  • Immunoglobulin E / immunology
  • Olea / adverse effects
  • Olea / genetics
  • Olea / immunology
  • Pollen / adverse effects
  • Pollen / genetics
  • Pollen / immunology
  • Proteome / genetics*
  • Proteome / immunology
  • Proteomics
  • Tandem Mass Spectrometry

Substances

  • Allergens
  • Antigens, Plant
  • Proteome
  • Immunoglobulin E
  • Cyclophilins