Hepatitis E virus prevalence in Flemish blood donors

J Viral Hepat. 2019 Oct;26(10):1218-1223. doi: 10.1111/jvh.13161. Epub 2019 Jul 24.


Transmission of hepatitis E virus (HEV) through transfusion of blood components has already been reported in several European countries. Here, we assessed the HEV prevalence in Flemish blood donors. This study is of importance in order to assess the risk of HEV transmission through blood transfusion. We analysed 38 137 blood donation samples that were collected by the Red Cross Flanders during the period May-June 2015. All samples were screened for the presence of HEV RNA and a selection for HEV-specific IgM/IgG. After pooling per 6, 11 pools reacted positive during RNA screening. Reactive pools were deconstructed, and individual samples were retested. After deconstruction, seven samples were confirmed as HEV RNA positive. Serological screening of the confirmed RNA-positive samples showed that six out of these seven samples were HEV IgM positive, of which three donors were also IgG positive. Serological screening was also performed on the samples that constituted the four initially HEV RNA reactive pools where RNA positivity was not confirmed on the individual level. In three pools, we found indirect evidence of recent HEV exposure. Within 356 randomly selected samples, 31 donations were HEV IgG positive. Here we show that at least 1:5448 of blood donations in Flanders may originate from donors that are actively infected with HEV. Upon transfusion, these donations may pose a major threat towards patients at risk. Finally, a serological analysis showed that the anti-HEV IgG prevalence in Flemish blood donors is 8.71%.

Keywords: blood donors; hepatitis E virus; transmission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Belgium / epidemiology
  • Blood Donors*
  • Female
  • Hepatitis Antibodies / blood
  • Hepatitis E / epidemiology*
  • Hepatitis E virus / isolation & purification
  • Humans
  • Immunoglobulin G / blood
  • Male
  • Middle Aged
  • Prevalence
  • RNA, Viral / blood
  • Sequence Analysis, RNA
  • Young Adult


  • Hepatitis Antibodies
  • Immunoglobulin G
  • RNA, Viral