Background: Sutures which are used for wound approximation can act as a reservoir of microbes at the surgical site leading to increased chances of surgical site infection (SSI). Sutures used in oral cavity are continuously bathed in saliva which results in wicking. Several studies on sutures treated with nanoparticles, antibacterial agent and various drugs to advance the therapeutical value of surgical sutures are in consideration, drug-eluting sutures has been notable in research to deliver localized effect on the site of incision. Ciprofloxacin and Aloe vera are routinely used agents in coating sutures.
Aim: This study is to evaluate the antibacterial efficacy and oral biofilm inhibition of Ciprofloxacin and Aloe vera coated 3-0 silk sutures in comparison to uncoated sutures against E.coli.
Material and methods: Equal segments of ciprofloxacin and aloe vera coated 3-0 silk sutures are to be incubated in E.coli culture media (blood agar) at 37°C for 24 hours in aerobic atmosphere. Plain uncoated suture served as control. Assessment was done using Total Colony Forming Units and biofilm inhibition potential of sutures. Results awaited.
Results: The zone of inhibition around ciprofloxacin coated suture is nearly double than that of with Aloe vera indicted that antibacterial efficacy of ciprofloxacin is more comparatively. No inhibition zone around uncoated plain 3-0 braided silk shows that it has no significant antibacterial activity.
Conclusion: Within limitation of our study, it can be concluded that both ciprofloxacin and Aloe vera coated sutures have antibacterial property against gram negative E. coli and can have a promising role in prevention of SSI although it would require further in vivo validation.
Keywords: Aloe vera; E. coli; antibacterial sutures; ciprofloxacin.
Conflict of interest statement
There are no conflicts of interest.
Comparative evaluation of sutures coated with triclosan and chlorhexidine for oral biofilm inhibition potential and antimicrobial activity against periodontal pathogens: An in vitro study.Indian J Dent Res. 2016 Sep-Oct;27(5):535-539. doi: 10.4103/0970-9290.195644. Indian J Dent Res. 2016. PMID: 27966513
New Biofunctional Loading of Natural Antimicrobial Agent in Biodegradable Polymeric Films for Biomedical Applications.Int J Biomater. 2016;2016:6964938. doi: 10.1155/2016/6964938. Epub 2016 Nov 14. Int J Biomater. 2016. PMID: 27965710 Free PMC article.
Bacterial adherence to surgical sutures: can antibacterial-coated sutures reduce the risk of microbial contamination?J Am Coll Surg. 2006 Oct;203(4):481-9. doi: 10.1016/j.jamcollsurg.2006.06.026. Epub 2006 Aug 22. J Am Coll Surg. 2006. PMID: 17000391
Efficacy of triclosan-coated sutures for reducing risk of surgical site infection in adults: a meta-analysis of randomized clinical trials.J Surg Res. 2016 Mar;201(1):105-17. doi: 10.1016/j.jss.2015.10.015. Epub 2015 Oct 23. J Surg Res. 2016. PMID: 26850191 Review.
Impact of intra-abdominal absorbable sutures on surgical site infection in gastrointestinal and hepato-biliary-pancreatic surgery: results of a multicenter, randomized, prospective, phase II clinical trial.Surg Today. 2017 Sep;47(9):1060-1071. doi: 10.1007/s00595-017-1480-3. Epub 2017 Feb 23. Surg Today. 2017. PMID: 28233105 Review.
- Jacob S. Global prevalence of periodontitis. A literature review. Int Arab J Dent. 2012;3:25–30.
- Heitz-Mayfield LJ, Trombelli L, Heitz F, Needleman I, Moles D. A systematic review of the effect of surgical debridement vs non-surgical debridement for the treatment of chronic periodontitis. J Clin Periodontol. 2002;29(Suppl 3):92–102. discussion 160–2. - PubMed
- Mangram AJ, Horan TC, Pearson ML, Silver LC, Jarvis WR. Guideline for prevention of surgical site infection, 1999. Centers for disease control and prevention (CDC) hospital infection control practices advisory committee. Am J Infect Control. 1999;27:97–132. quiz 133–4; discussion 96. - PubMed
- Sunitha S, Adinarayana K, Pankaj T, Sravanthi Reddy P, Sonia G, Nagarjun R, et al. Fabrication of surgical sutures coated with Curcumin loaded gold nanoparticles. Pharm Anal Acta. 2017;8:529.