Dietary Alaska pollack protein improves skeletal muscle weight recovery after immobilization-induced atrophy in rats

Mina Fujitani; Takafumi Mizushige; Fuminori Kawabata; Keisuke Uozumi; Machi Yasui; Kohsuke Hayamizu; Kenji Uchida; Shinji Okada; Bhattarai Keshab; Taro Kishida

doi:10.1371/journal.pone.0217917

Dietary Alaska pollack protein improves skeletal muscle weight recovery after immobilization-induced atrophy in rats

PLoS One. 2019 Jun 14;14(6):e0217917. doi: 10.1371/journal.pone.0217917. eCollection 2019.

Authors

Affiliations

¹ Laboratory of Nutrition Science, Division of Applied bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Japan.
² Department of Applied Biological Chemistry, Faculty of Agriculture, Utsunomiya University, Minemachi, Utsunomiya, Tochigi, Japan.
³ Physiology of Domestic Animals, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho, Hirosaki, Aomori, Japan.
⁴ Laboratory of Food Chemistry, Yokohama University of Pharmacy, Yokohama, Japan.
⁵ Food Function R&D Center, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
⁶ Graduate School of Agricultural and Life Sciences, the University of Tokyo, Tokyo, Japan.
⁷ Food and Health Sciences Research Centre, Graduate School of Agriculture, Ehime University, Matsuyama, Japan.

Abstract

The promotion of muscle recovery after immobilization is important to preserve an optimum health status. Here, we examined the effect of dietary Alaska pollack protein (APP) on skeletal muscle weight after atrophy induced by hind limb immobilization using plaster immobilization technique. Rat left limb was casted with a wetted plaster cast under anesthesia. After 2 weeks of feeding, the cast was removed and the rats were divided into three groups, namely, a baseline group, high-fat casein diet group, and high-fat APP diet group. After 3 weeks of feeding, the skeletal muscles (soleus, extensor digitorum longus [EDL], and gastrocnemius) were sampled. The estimated weight gains of soleus, gastrocnemius, and EDL muscle in the immobilized limbs were significantly larger in the rats fed with APP diet as compared with those fed with casein diet. In soleus muscle, dietary APP increased the expression of Igf1 and Myog genes in the immobilized limbs after the recovery period.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alaska
Animals
Casts, Surgical
Extremities / physiopathology
Fish Proteins, Dietary / pharmacology*
Immobilization / physiology*
Insulin-Like Growth Factor I / metabolism
Male
Muscle Proteins / metabolism
Muscle, Skeletal / drug effects*
Muscular Atrophy / drug therapy*
Rats
Rats, Sprague-Dawley

Substances

Fish Proteins, Dietary
Muscle Proteins
Insulin-Like Growth Factor I

Grants and funding

This work was supported by Japanese Council for Science, Technology and Innovation (CSTI), Cross-ministerial Strategic Innovation Promotion Program (SIP Project ID 14533567). These funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This study was funded by Nippon Suisan Kaisha, Ltd. This funder provided support in the form of salaries for author KU and research materials, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.