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Case Reports
. 2019 Jun 13;6(2):56.
doi: 10.3390/vetsci6020056.

Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey

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Free PMC article
Case Reports

Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey

Harun Albayrak et al. Vet Sci. .
Free PMC article

Abstract

A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies.

Keywords: Bovine parainfluenza virus type 3; Turkey.

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Cytopatheic effect induced by the Bovine parainfluenza virus (BPIV)_3c isolate on MDBK cells 300 dpi. (Magnification: 400x). A: Infected cells; large areas of the monolayer have lysed. B: negative control.
Figure 2
Figure 2
Phylogenetic tree of published full genome sequences of BPIV-3 strains. Strains are identified by their accession numbers. High bootstrap values are indicated. The Turkish isolate is highlighted in blue (Genebank accession no. MH357343). Chinese isolates are highlighted in red.

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