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Comparative Study
, 238 (4830), 1134-8

Two Pairs of Recombination Signals Are Sufficient to Cause Immunoglobulin V-(D)-J Joining

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Comparative Study

Two Pairs of Recombination Signals Are Sufficient to Cause Immunoglobulin V-(D)-J Joining

S Akira et al. Science.

Abstract

The minimum sequence requirements for antigen receptor V-(D)-J joining were studied by constructing recombination-substrates containing synthetic recombination signals and introducing them into a recombination-competent pre-B cell line. Two sets of heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences were shown to be sufficient to cause the V-(D)-J joining, if the 12- and 23-base pair spacer rule is satisfied. A point mutation in the heptamer sequence, or a change in the combination of the two spacer lengths, drastically reduced the recombination.

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