Arabidopsis EMSY-like (EML) histone readers are necessary for post-fertilization seed development, but prevent fertilization-independent seed formation

Plant Sci. 2019 Aug:285:99-109. doi: 10.1016/j.plantsci.2019.04.007. Epub 2019 Apr 24.

Abstract

Seed development is a complex regulatory process that includes a transition from gametophytic to sporophytic program. The synchronized development of different seed compartments (seed coat, endosperm and embryo) depends on a balance in parental genome contributions. In the most severe cases, the disruption of the balance leads to seed abortion. This represents one of the main obstacles for the engineering of asexual reproduction through seeds (apomixis), and for generating new interspecies hybrids. The repression of auxin synthesis by the Polycomb Repressive Complex 2 (PRC2) is a major mechanism contributing to sensing genome balance. However, current efforts focusing on decreasing PRC2 or elevating auxin levels have not yet resulted in the production of apomictic seed. Here, we show that EMSY-Like Tudor/Agenet H3K36me3 histone readers EML1 and EML3 are necessary for early stages of seed development to proceed at a normal rate in Arabidopsis. We further demonstrate that both EML1 and EML3 are required to prevent the initiation of seed development in the absence of fertilization. Based on the whole genome expression analysis, we identify auxin transport and signaling genes as the most enriched downstream targets of EML1 and EML3. We hypothesize that EML1 and EML3 function to repress and soften paternal gene expression by fine-tuning auxin responses. Discovery of this pathway may contribute to the engineering of apomixis and interspecies hybrids.

Keywords: Apomixis; Arabidopsis; Auxin; EML; Histone readers; Seed development.

MeSH terms

  • Apomixis
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / physiology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / physiology*
  • Fertilization
  • Histones / metabolism*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / physiology*
  • Phylogeny
  • Plants, Genetically Modified
  • Polymerase Chain Reaction
  • Seeds / growth & development*
  • Seeds / physiology

Substances

  • AT3G12140 protein, Arabidopsis
  • AT5G13020 protein, Arabidopsis
  • Arabidopsis Proteins
  • Cytoskeletal Proteins
  • Histones
  • Nuclear Proteins