A novel subtype of muscarinic receptor identified by homology screening

T Braun; P R Schofield; B D Shivers; D B Pritchett; P H Seeburg

doi:10.1016/0006-291x(87)91613-5

A novel subtype of muscarinic receptor identified by homology screening

Biochem Biophys Res Commun. 1987 Nov 30;149(1):125-32. doi: 10.1016/0006-291x(87)91613-5.

Authors

T Braun¹, P R Schofield, B D Shivers, D B Pritchett, P H Seeburg

Affiliation

¹ Laboratory of Molecular Neurobiology, ZMBH, University of Heidelberg, F.R.G.

PMID: 3120722
DOI: 10.1016/0006-291x(87)91613-5

Abstract

A new member of the protein superfamily of G-protein coupled receptors has been isolated by homology screening. By virtue of its homology with other muscarinic acetylcholine receptors and its ability to bind muscarinic specific antagonists, this muscarinic receptor subtype is designated M4. The M4 mRNA is preferentially expressed in certain brain regions. The existence of multiple receptor subtypes encoded by distinct genes in the brain has functional implications for the molecular mechanisms underlying information transmission in neuronal networks.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence*
Brain / metabolism
DNA / genetics
DNA, Recombinant
GTP-Binding Proteins / metabolism
Molecular Sequence Data
Nucleic Acid Hybridization
Quinuclidinyl Benzilate / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Receptors, Muscarinic / genetics*
Receptors, Muscarinic / metabolism
Sequence Homology, Nucleic Acid*
Tissue Distribution

Substances

DNA, Recombinant
RNA, Messenger
Receptors, Muscarinic
Quinuclidinyl Benzilate
DNA
GTP-Binding Proteins

Associated data

GENBANK/M18088