Teburu-Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models

Artif Organs. 2019 Oct;43(10):1035-1041. doi: 10.1111/aor.13518. Epub 2019 Jul 17.


Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.

Keywords: 3D cell culture; 3D-printing; bioreactor; optogenetics; recellularization.

MeSH terms

  • Bioprinting*
  • Bioreactors*
  • Equipment Design
  • Humans
  • Liver / chemistry
  • Liver / cytology
  • Liver / ultrastructure
  • Printing, Three-Dimensional*
  • Tissue Culture Techniques / instrumentation*
  • Tissue Engineering / instrumentation
  • Tissue Scaffolds / chemistry