RNA-induced initiation of transcriptional silencing (RITS) complex structure and function

RNA Biol. 2019 Sep;16(9):1133-1146. doi: 10.1080/15476286.2019.1621624. Epub 2019 Jun 18.


Heterochromatic regions of the genome are epigenetically regulated to maintain a heritable '"silent state"'. In fission yeast and other organisms, epigenetic silencing is guided by nascent transcripts, which are targeted by the RNA interference pathway. The key effector complex of the RNA interference pathway consists of small interfering RNA molecules (siRNAs) associated with Argonaute, assembled into the RNA-induced transcriptional silencing (RITS) complex. This review focuses on our current understanding of how RITS promotes heterochromatin formation, and in particular on the role of Argonaute-containing complexes in many other functions such as quelling, release of RNA polymerases, cellular quiescence and genome defense.

Keywords: DNA repair; RITS; RNA; RNAi; argonaute; cell cycle; dicer; epigenetics; genome defense; quelling; quiescence; silencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Argonaute Proteins / chemistry
  • Argonaute Proteins / genetics*
  • Cell Cycle / genetics
  • Genome, Fungal / genetics
  • Multiprotein Complexes / chemistry
  • Multiprotein Complexes / genetics
  • RNA / chemistry*
  • RNA / genetics
  • RNA / ultrastructure
  • RNA, Small Interfering / chemistry*
  • RNA, Small Interfering / genetics
  • RNA-Induced Silencing Complex / chemistry*
  • RNA-Induced Silencing Complex / genetics
  • RNA-Induced Silencing Complex / ultrastructure
  • Schizosaccharomyces / genetics


  • Argonaute Proteins
  • Multiprotein Complexes
  • RNA, Small Interfering
  • RNA-Induced Silencing Complex
  • RNA