Abstract
We report the first dual-responsive 19F MRI and fluorescence imaging probe for cellular hypoxia. The Cu2+-based probe exhibits no 19F MR signal and reduced fluorescence signal due to paramagnetic quenching; however, the probe turns-on in both modes following reduction to Cu+. This bimodal agent can differentiate hypoxic and normoxic cells in both modalities.
MeSH terms
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Cell Hypoxia / physiology*
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Coordination Complexes / chemical synthesis
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Coordination Complexes / chemistry*
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Coordination Complexes / toxicity
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Copper / chemistry
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Fluoresceins / chemical synthesis
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Fluoresceins / chemistry*
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Fluoresceins / toxicity
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Fluorescence
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / toxicity
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Fluorine
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Fluorine-19 Magnetic Resonance Imaging / methods
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HeLa Cells
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Humans
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Hydrogen-Ion Concentration
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Microscopy, Fluorescence / methods
Substances
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Coordination Complexes
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Fluoresceins
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Fluorescent Dyes
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Fluorine
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Copper