A dual-responsive probe for detecting cellular hypoxia using 19F magnetic resonance and fluorescence

Chem Commun (Camb). 2019 Jul 23;55(60):8860-8863. doi: 10.1039/c9cc00375d.

Abstract

We report the first dual-responsive 19F MRI and fluorescence imaging probe for cellular hypoxia. The Cu2+-based probe exhibits no 19F MR signal and reduced fluorescence signal due to paramagnetic quenching; however, the probe turns-on in both modes following reduction to Cu+. This bimodal agent can differentiate hypoxic and normoxic cells in both modalities.

MeSH terms

  • Cell Hypoxia / physiology*
  • Coordination Complexes / chemical synthesis
  • Coordination Complexes / chemistry*
  • Coordination Complexes / toxicity
  • Copper / chemistry
  • Fluoresceins / chemical synthesis
  • Fluoresceins / chemistry*
  • Fluoresceins / toxicity
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / toxicity
  • Fluorine
  • Fluorine-19 Magnetic Resonance Imaging / methods
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Microscopy, Fluorescence / methods

Substances

  • Coordination Complexes
  • Fluoresceins
  • Fluorescent Dyes
  • Fluorine
  • Copper