Intracellular injection of a Ca2+ chelator inhibits spike repolarization in hippocampal neurons

Brain Res. 1987 Dec 1;435(1-2):387-92. doi: 10.1016/0006-8993(87)91631-3.


The Ca-dependence of spike repolarization and afterhyperpolarizations (AHPs) in Ca1 pyramidal cells, was tested with intracellular electrodes containing the Ca buffers EGTA or 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). EGTA blocked only the slow AHP; but the fast-acting Ca chelator BAPTA also inhibited spike repolarization and the fast AHP. This supports the hypothesis that a fast Ca-activated'K-current contributes to spike repolarization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / pharmacology*
  • Chelating Agents / pharmacology*
  • Egtazic Acid / pharmacology*
  • Evoked Potentials / drug effects
  • Hippocampus / drug effects
  • Hippocampus / physiology*
  • Neurons / drug effects
  • Neurons / physiology*
  • Pyramidal Tracts / drug effects
  • Pyramidal Tracts / physiology
  • Rats


  • Chelating Agents
  • Egtazic Acid
  • 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
  • Calcium