Production and Culture of the Bovine Embryo

Methods Mol Biol. 2019;2006:115-129. doi: 10.1007/978-1-4939-9566-0_8.

Abstract

A protocol for production of bovine embryos from oocytes collected from ovaries obtained from an abattoir is described. The protocol includes methods for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.

Keywords: Bovine; Culture; Embryo; In vitro fertilization; In vitro maturation.

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism*
  • Cattle
  • Embryo Culture Techniques / methods*
  • Female
  • Fertilization in Vitro / methods*
  • In Vitro Oocyte Maturation Techniques / methods*
  • Male
  • Oocytes / cytology
  • Oocytes / metabolism*
  • Spermatozoa / cytology
  • Spermatozoa / metabolism*