The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer

J B Nagashima; A J Travis; N Songsasen

doi:10.1007/978-1-4939-9566-0_18

The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer

Methods Mol Biol. 2019:2006:247-267. doi: 10.1007/978-1-4939-9566-0_18.

Authors

J B Nagashima¹, A J Travis^{2

3}, N Songsasen⁴

Affiliations

¹ Smithsonian Conservation Biology Institute, Front Royal, VA, USA. nagashimaj@si.edu.
² Baker Institute for Animal Health, College of Veterinary Medicine, Ithaca, NY, USA.
³ Atkinson Center for a Sustainable Future, Cornell University, Ithaca, NY, USA.
⁴ Smithsonian Conservation Biology Institute, Front Royal, VA, USA.

PMID: 31230286
DOI: 10.1007/978-1-4939-9566-0_18

Abstract

Advances in embryo technologies in the domestic dog have made significant strides in the past decade. This progress has been spurred by interests in taking advantage of the dog as a biomedical research model for human and companion animal medicine, developing assisted reproductive technologies to manage genetic diversity in endangered canids maintained ex situ, and improving breeding in rare or working breeds of dogs. Here, we focus on recent advancements and techniques for collection of in vivo-matured oocytes, in vitro fertilization (IVF), in vitro culture of early (≤8-cell) and advanced stage (≥16-cell) embryos, and embryo transfer.

Keywords: Domestic dog; Embryo cleavage kinetics; Embryo culture; In vitro fertilization; Oocyte.

MeSH terms

Animals
Dogs
Embryo Culture Techniques / methods*
Embryo Transfer / methods*
Embryo, Mammalian / cytology
Embryo, Mammalian / embryology*
Female
Fertilization in Vitro / methods*
In Vitro Oocyte Maturation Techniques / methods*
Oocytes / cytology
Oocytes / metabolism*