Rmrp Mutation Disrupts Chondrogenesis and Bone Ossification in Zebrafish Model of Cartilage-Hair Hypoplasia via Enhanced Wnt/β-Catenin Signaling

J Bone Miner Res. 2019 Nov;34(11):2101-2116. doi: 10.1002/jbmr.3820. Epub 2019 Sep 4.

Abstract

Cartilage-hair hypoplasia (CHH) is an autosomal recessive metaphyseal chondrodysplasia characterized by bone dysplasia and many other highly variable features. The gene responsible for CHH is the RNA component of the mitochondrial RNA-processing endoribonuclease (RMRP) gene. Currently, the pathogenesis of osteochondrodysplasia and extraskeletal manifestations in CHH patients remains incompletely understood; in addition, there are no viable animal models for CHH. We generated an rmrp KO zebrafish model to study the developmental mechanisms of CHH. We found that rmrp is required for the patterning and shaping of pharyngeal arches. Rmrp mutation inhibits the intramembranous ossification of skull bones and promotes vertebrae ossification. The abnormalities of endochondral bone ossification are variable, depending on the degree of dysregulated chondrogenesis. Moreover, rmrp mutation inhibits cell proliferation and promotes apoptosis through dysregulating the expressions of cell-cycle- and apoptosis-related genes. We also demonstrate that rmrp mutation upregulates canonical Wnt/β-catenin signaling; the pharmacological inhibition of Wnt/β-catenin could partially alleviate the chondrodysplasia and increased vertebrae mineralization in rmrp mutants. Our study, by establishing a novel zebrafish model for CHH, partially reveals the underlying mechanism of CHH, hence deepening our understanding of the role of rmrp in skeleton development.

Keywords: CARTILAGE-HAIR HYPOPLASIA; RMRP; SKELETAL DEVELOPMENT; WNT/Β-CATENIN; ZEBRAFISH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chondrogenesis / genetics*
  • Disease Models, Animal
  • Hair / abnormalities*
  • Hair / metabolism
  • Hair / pathology
  • Hirschsprung Disease* / genetics
  • Hirschsprung Disease* / metabolism
  • Hirschsprung Disease* / pathology
  • Humans
  • Mutation*
  • Osteochondrodysplasias / congenital*
  • Osteochondrodysplasias / genetics
  • Osteochondrodysplasias / metabolism
  • Osteochondrodysplasias / pathology
  • Osteogenesis / genetics*
  • Primary Immunodeficiency Diseases* / genetics
  • Primary Immunodeficiency Diseases* / metabolism
  • Primary Immunodeficiency Diseases* / pathology
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • Skull / metabolism
  • Skull / pathology
  • Spine / metabolism
  • Spine / pathology
  • Wnt Signaling Pathway / genetics*
  • Zebrafish / metabolism*

Substances

  • RNA, Long Noncoding

Supplementary concepts

  • Cartilage-hair hypoplasia