BMP2 and TGF-β Cooperate Differently during Synovial-Derived Stem-Cell Chondrogenesis in a Dexamethasone-Dependent Manner

Cells. 2019 Jun 25;8(6):636. doi: 10.3390/cells8060636.

Abstract

Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.

Keywords: BMP-2; TGF-β; chondrogenesis; dexamethasone; synovial derived stromal cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bone Morphogenetic Protein 2 / pharmacology*
  • Chondrogenesis / drug effects*
  • Collagen Type II / metabolism
  • DNA / metabolism
  • Dexamethasone / pharmacology*
  • Gene Expression Regulation / drug effects
  • Glycosaminoglycans / metabolism
  • Humans
  • Male
  • Middle Aged
  • Stem Cells / cytology*
  • Synovial Membrane / cytology*
  • Transforming Growth Factor beta1 / pharmacology*
  • Young Adult

Substances

  • Bone Morphogenetic Protein 2
  • Collagen Type II
  • Glycosaminoglycans
  • Transforming Growth Factor beta1
  • Dexamethasone
  • DNA