Effectiveness of Enzyme Dentifrices on Oral Health in Orthodontic Patients: A Randomized Controlled Trial

Int J Environ Res Public Health. 2019 Jun 25;16(12):2243. doi: 10.3390/ijerph16122243.


Plaque accumulation and white spot lesions are common adverse effects of fixed orthodontic appliance use. This study compared the effects between enzyme-containing and conventional dentifrices on orthodontic patients. This double-blind randomized controlled trial included 42 orthodontic patients (25 women and 17 men: 22.7 ± 4.2 years) from Taipei Medical University Hospital between 2017 and 2018. The patients were randomly divided into three groups and assigned to dentifrice use during the first 3 months of the orthodontic treatment: group 1 used dentifrices containing enzymes including amyloglucosidase and glucose oxidase, group 2 used dentifrices containing 1450 ppm fluoride, and group 3 used natural dentifrices containing no chemical agent. White spot lesion index (WSL), gingival bleeding index (GBI), and visible plaque index (VPI) were recorded and analyzed. WSL, GBI, and VPI values exhibited no significant difference among the three groups. WSL increased significantly in group 3, GBI decreased significantly in all groups, and VPI decreased significantly in groups 1 and 2. No significant difference was observed between the use of enzyme-containing and conventional dentifrices after fixed orthodontic appliance placement.

Keywords: dental care; dentifrice; enzyme; fluoride; oral health literacy; orthodontic patient.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Dental Plaque Index
  • Dentifrices / therapeutic use*
  • Double-Blind Method
  • Female
  • Fluorides / therapeutic use*
  • Gingivitis
  • Glucan 1,4-alpha-Glucosidase / therapeutic use*
  • Glucose Oxidase / therapeutic use*
  • Humans
  • Male
  • Oral Health
  • Orthodontic Appliances
  • Periodontal Index
  • Young Adult


  • Dentifrices
  • Glucose Oxidase
  • Glucan 1,4-alpha-Glucosidase
  • Fluorides