Four amino acids define the CO 2 binding pocket of enoyl-CoA carboxylases/reductases

Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):13964-13969. doi: 10.1073/pnas.1901471116. Epub 2019 Jun 26.


Carboxylases are biocatalysts that capture and convert carbon dioxide (CO2) under mild conditions and atmospheric concentrations at a scale of more than 400 Gt annually. However, how these enzymes bind and control the gaseous CO2 molecule during catalysis is only poorly understood. One of the most efficient classes of carboxylating enzymes are enoyl-CoA carboxylases/reductases (Ecrs), which outcompete the plant enzyme RuBisCO in catalytic efficiency and fidelity by more than an order of magnitude. Here we investigated the interactions of CO2 within the active site of Ecr from Kitasatospora setae Combining experimental biochemistry, protein crystallography, and advanced computer simulations we show that 4 amino acids, N81, F170, E171, and H365, are required to create a highly efficient CO2-fixing enzyme. Together, these 4 residues anchor and position the CO2 molecule for the attack by a reactive enolate created during the catalytic cycle. Notably, a highly ordered water molecule plays an important role in an active site that is otherwise carefully shielded from water, which is detrimental to CO2 fixation. Altogether, our study reveals unprecedented molecular details of selective CO2 binding and C-C-bond formation during the catalytic cycle of nature's most efficient CO2-fixing enzyme. This knowledge provides the basis for the future development of catalytic frameworks for the capture and conversion of CO2 in biology and chemistry.

Keywords: CO2 fixation; RuBisCO; carbon dioxide; carboxylases; enzyme mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acids / chemistry*
  • Amino Acids / genetics
  • Amino Acids / metabolism
  • Carbon Dioxide / chemistry*
  • Carbon Dioxide / metabolism
  • Carrier Proteins / chemistry
  • Catalysis
  • Catalytic Domain / genetics
  • Enzymes / chemistry
  • Fatty Acid Desaturases / chemistry*
  • Fatty Acid Desaturases / metabolism
  • Models, Molecular*
  • Streptomycetaceae / chemistry
  • Streptomycetaceae / enzymology


  • Amino Acids
  • Carrier Proteins
  • Enzymes
  • Carbon Dioxide
  • Fatty Acid Desaturases

Supplementary concepts

  • Kitasatospora setae

Associated data

  • PDB/6OWE