Terbium(III) Luminescence-Based Assay for Esterase Activity

Anal Chem. 2019 Jul 2;91(13):8615-8621. doi: 10.1021/acs.analchem.9b01954. Epub 2019 Jun 18.

Abstract

Esterases catalyze the hydrolysis of esters to form a carboxylic acid and alcohol. These enzymes play a key role in both the detoxification of xenobiotic compounds and the metabolism of drugs and prodrugs. Numerous fluorogenic probes have been developed to monitor esterase activity. Most are based on an aromatic alcohol, and the others are based on an aromatic acid. These restrictions leave unexplored the specificity of esterases for aliphatic esters. Here, we report on the use of esters of thiopheneacetic acid coupled with the luminescence of terbium(III) as the basis for a continuous assay of esterase activity. This probe allows for a wide variation of the alcohol moiety and the detection of its hydrolysis at submicromolar concentrations. The assay verifies steady-state kinetic parameters for catalysis by pig liver esterase from either initial rates or the integration of progress curves, and its utility is evident with unpurified esterases in bacterial and human cell lysates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillus subtilis / enzymology*
  • Catalysis
  • Esterases / metabolism*
  • Esters / metabolism*
  • Fluorescent Dyes / chemistry*
  • HEK293 Cells
  • Humans
  • Hydrolysis
  • Liver / enzymology*
  • Luminescent Measurements / methods*
  • Substrate Specificity
  • Swine
  • Terbium / chemistry*

Substances

  • Esters
  • Fluorescent Dyes
  • Terbium
  • Esterases