The Genome of the Steller Sea Lion (Eumetopias jubatus)

Genes (Basel). 2019 Jun 26;10(7):486. doi: 10.3390/genes10070486.

Abstract

The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.

Keywords: Eumetopias jubatus; Steller sea lion; genome; marine animal; microfluidic partitioning; nanopore.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Genome*
  • Genomic Library
  • Microfluidics / methods
  • Nanopores
  • Sea Lions / genetics*
  • Whole Genome Sequencing