Formation of lipoxins by alveolar macrophages

Biochem Biophys Res Commun. 1988 Jan 29;150(2):870-6. doi: 10.1016/0006-291x(88)90473-1.

Abstract

Rat alveolar macrophages incubated with (15S), 15-hydroperoxy-5,8, 11-cis-13-trans-eicosatetraenoic acid (15-HPETE) in the presence of the calcium ionophore A23187 produced a series of polar compounds. Reverse-phase high performance liquid chromatography (RP-HPLC) was used to purify these compounds and their identities were characterized by physical criteria (UV spectroscopy, GC/MS) and by co-elution with synthetic compounds. Alveolar macrophage-derived materials proved to be lipoxin A4 [LXA4; (5S, 6R, 15S)-5,6,15-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid], lipoxin B4[LXB4; (5S, 14R, 15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis- eicosatetraenoic acid], and a structural isomer. These results indicate that rat alveolar macrophages have the capacity to generate lipoxins and related compounds.

MeSH terms

  • Animals
  • Arachidonic Acids / metabolism*
  • Calcimycin / pharmacology
  • Chromatography, High Pressure Liquid
  • Gas Chromatography-Mass Spectrometry
  • Hydroxyeicosatetraenoic Acids / biosynthesis*
  • Hydroxyeicosatetraenoic Acids / isolation & purification
  • In Vitro Techniques
  • Leukotrienes*
  • Lipid Peroxides / metabolism*
  • Macrophages / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Spectrophotometry, Ultraviolet

Substances

  • Arachidonic Acids
  • Hydroxyeicosatetraenoic Acids
  • Leukotrienes
  • Lipid Peroxides
  • Calcimycin
  • 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid