Wogonoside promotes apoptosis in gastric cancer AGS and SGC-7901 cells through induction of mitochondrial dysfunction and endoplasmic reticulum stress

FEBS Open Bio. 2019 Aug;9(8):1469-1476. doi: 10.1002/2211-5463.12693. Epub 2019 Jul 17.

Abstract

Wogonoside (Wg), a natural flavonoid, has anticancer effects against several human cancers. The purpose of the present study was to investigate the antitumor effects and underlying mechanisms of Wg on gastric cancer (GC) cell lines. We report that Wg treatment inhibited cell viability and induced apoptosis in human GC cell lines AGS and SGC-7901, and also retarded GC tumor growth in xenograft mice in vivo. We also found that the Wg exerted its antitumor effects against GC cells via induction of reaction oxygen species accumulation, mitochondrial dysfunction, and endoplasmic reticulum stress. Furthermore, C/EBP homologous protein knockdown inhibited apoptosis and increased the viability of Wg-treated GC cells. Our findings may facilitate the development of novel therapeutic agents for the treatment of GC.

Keywords: endoplasmic reticulum stress; gastric cancer; mitochondrial dysfunction; wogonoside.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cell Line, Tumor / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Endoplasmic Reticulum Stress / drug effects
  • Flavanones / pharmacology*
  • Glucosides / pharmacology*
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Mitochondria / drug effects
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • Stomach Neoplasms / drug therapy*
  • Stomach Neoplasms / metabolism
  • Xenograft Model Antitumor Assays / methods

Substances

  • Flavanones
  • Glucosides
  • Reactive Oxygen Species
  • wogonoside