Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p

Clin Res Hepatol Gastroenterol. 2020 Feb;44(1):101-114. doi: 10.1016/j.clinre.2019.05.001. Epub 2019 Jun 26.


Background/aims: Colon cancer is a common cancer that is a threat to human health. Some long non-coding RNAs (lncRNAs) have been observed to exert roles in colon cancer. Here, the current study is aimed to explore the potential mechanism of lncRNA MBNL1 antisense RNA 1 (MBNL1-AS1) in progression of colon cancer and the associated mechanisms.

Methods: Microarray analysis was performed to screen differentially expressed lncRNA and genes associated with colon cancer and its potential mechanism. The functional role of MBNL1-AS1 in colon cancer was analyzed, followed identification of the interaction among MBNL1-AS1, microRNA-412-3p (miR-412-3p), and MYL9. Subsequently, CSC viability, migration, invasion, and apoptosis were detected though a series of in vitro experiments. At last, in vivo experiments were performed to assess tumor formation of colon CSCs.

Results: MBNL1-AS1 and MYL9 were poorly expressed in colon cancer. MBNL1-AS1 could competitively bind to miR-412-3p so as to promote MYL9 expression. Enhancement of MBNL1-AS1 or inhibition of miR-412-3p was shown to decrease CSC proliferation, migration, and invasion but promote apoptosis. Moreover, MBNL1-AS1 reversed the CSC-like properties as well as xenograft tumor formation in vivo induced by miR-412-3p.

Conclusion: Collectively, the present study suggests an inhibitory role of MBNL1-AS1 in colon cancer by upregulating miR-412-3p-targeted MYL9. Thus, this study provides an enhanced understanding of MBNL1-AS1 along with miR-412-3p and MYL9 as therapeutic targets for colon cancer.

Keywords: Cancer stem cells; Colon Cancer; Long non-coding RNA MBNL1-AS1; MYL9; MicroRNA-412-3p.

MeSH terms

  • Cell Movement*
  • Cell Proliferation*
  • Colonic Neoplasms / pathology*
  • Humans
  • MicroRNAs / physiology*
  • Myosin Light Chains / physiology*
  • Neoplasm Invasiveness
  • Neoplastic Stem Cells / physiology*
  • RNA, Long Noncoding
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / physiology*
  • Tumor Cells, Cultured


  • MBNL1 protein, human
  • MIRN412 microRNA, human
  • MYL9 protein, human
  • MicroRNAs
  • Myosin Light Chains
  • RNA, Long Noncoding
  • RNA-Binding Proteins