Natriuretic peptide signaling is involved in the expression of oxidative metabolism-related and muscle fiber constitutive genes in the gastrocnemius muscle

Mol Cell Endocrinol. 2019 Aug 20;494:110493. doi: 10.1016/j.mce.2019.110493. Epub 2019 Jun 27.


Natriuretic peptides regulate cyclic guanosine monophosphate (cGMP) levels via their receptors and have various physiological effects. Natriuretic peptide receptor C (NPR-C) increases cGMP signaling by functioning as a clearance receptor. We analyzed the role of natriuretic peptides in the skeletal muscle, which increases in mass with bone elongation, of NPR-C- mice. High-fat diet (HFD)-fed NPR-C- mice exhibited obesity resistance and higher oxygen consumption. PGC1α gene expression was upregulated in the gastrocnemius muscle of HFD-fed NPR-C- mice compared with HFD-fed NPR-C+ (wild-type) mice. Gene expression of proliferator-activated receptor delta and estrogen-related receptor α, which upregulate oxidative metabolism, was increased in the gastrocnemius muscle of NPR-C- mice, irrespective of diet. Expression of myosin heavy chain 7, a component of type I slow-twitch fiber, was enhanced. Natriuretic peptide signaling may influence oxidative metabolism-related and slow-twitch fiber constitutive gene expression in the fast-twitch gastrocnemius muscle but not in slow-twitch muscles such as the soleus.

Keywords: Cyclic GMP; Gastrocnemius muscle; Metabolism; Myosin heavy chain; Natriuretic peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight
  • Diet, High-Fat
  • Gene Expression Regulation*
  • Image Processing, Computer-Assisted
  • Liver / metabolism
  • Mice, Inbred C57BL
  • Muscle Fibers, Skeletal / metabolism*
  • Natriuretic Peptides / metabolism*
  • Obesity / metabolism
  • Obesity / pathology
  • Organ Size
  • Oxidation-Reduction
  • Oxygen Consumption
  • Signal Transduction* / genetics
  • Up-Regulation / genetics


  • Natriuretic Peptides