Abstract
Genes in human chromosomes that normally require induction by alpha-interferon are activated after calcium phosphate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by gamma-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Calcimycin / pharmacology
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Calcium Phosphates / pharmacology*
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Clone Cells
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DNA / analogs & derivatives*
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DNA / pharmacology
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Gene Expression Regulation
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Genes / drug effects*
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HeLa Cells / metabolism
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Humans
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Interferon Type I / pharmacology
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Interferon-gamma / pharmacology
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Proto-Oncogenes / drug effects*
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RNA, Messenger / genetics
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Transcription, Genetic / drug effects*
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Transcriptional Activation
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Transfection
Substances
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Calcium Phosphates
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Interferon Type I
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RNA, Messenger
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calcium phosphate-DNA precipitate
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Calcimycin
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Interferon-gamma
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DNA