Abstract
A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.
MeSH terms
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Cell Line
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Cloning, Molecular
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DNA / analysis
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Escherichia coli / genetics*
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Exotoxins / genetics*
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Exotoxins / pharmacology
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Graft Rejection
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Humans
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Immunotoxins*
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Interleukin-2 / genetics*
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Interleukin-2 / pharmacology
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Plasmids
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Receptors, Immunologic / metabolism
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Receptors, Interleukin-2
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Recombinant Fusion Proteins / pharmacology*
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Recombinant Proteins / pharmacology*
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Transplantation, Homologous
Substances
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Exotoxins
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IL-2-PE40 chimeric protein, recombinant
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Immunotoxins
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Interleukin-2
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Receptors, Immunologic
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Receptors, Interleukin-2
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Recombinant Fusion Proteins
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Recombinant Proteins
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DNA