Correlation Between SEPS1 Gene Polymorphism and Type 2 Diabetes Mellitus: A Preliminary Study

J Clin Lab Anal. 2019 Oct;33(8):e22967. doi: 10.1002/jcla.22967. Epub 2019 Jul 2.

Abstract

Background: The protein encoded by the selenoprotein S gene is considered to be an anti-inflammatory and antioxidant protein and is involved in a variety of diseases. Therefore, we want to study the distribution characteristics of this gene in Chinese diabetic population.

Methods: A total of 170 patients with DM (including 100 patients with T2DM and 70 patients with diabetic nephropathy [DN]) and 100 healthy controls (HC) were selected from Haikou People's Hospital (China) between January 2017 and July 2017. The polymorphisms of three SEPS1 genes (SNP ID: rs4965814, rs28665122, and rs34713741) were measured by massARRAY method, while the polymorphisms of SEPS1 genes (SNP ID: rs4965373) were detected by Sanger sequencing.

Results: Comparing three groups, the results were the following: (a) There was a significant difference in the genotype and allele distribution of rs34713741 between DN group and HC group and between T2DM group and DN group; For this gene locus, the risk of diabetic nephropathy in healthy individuals with T allele was 0.6 times higher than that in individuals with GG genotype (OR = 0.60, 95% CI: 0.46 ~ 0.77). (b) There was a significant difference in the distribution of rs4975814 genotype between DN group and HC group; for this gene locus, the risk of diabetic nephropathy in healthy individuals with T allele was 2.71 times higher than that in individuals with GG genotype (OR = 2.71, 95% CI: 1.66 ~ 4.45).

Conclusion: We conclude that rs34713741 (GT + TT) may be a protective gene for DN and the rs4975814 (GT + TT) may be a susceptibility gene for DN.

Keywords: SEPS1 gene polymorphism; Type 2 diabetes mellitus; diabetic nephropathy; healthy controls.

MeSH terms

  • Adult
  • Asian Continental Ancestry Group / genetics*
  • Case-Control Studies
  • Diabetes Mellitus, Type 2 / complications*
  • Diabetes Mellitus, Type 2 / genetics
  • Diabetic Nephropathies / diagnosis*
  • Diabetic Nephropathies / etiology
  • Female
  • Follow-Up Studies
  • Genetic Predisposition to Disease*
  • Humans
  • Male
  • Membrane Proteins / genetics*
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Prognosis
  • Risk Factors
  • Selenoproteins / genetics*

Substances

  • Membrane Proteins
  • SELENOS protein, human
  • Selenoproteins