Purification of ribonuclease T1

C N Pace; G R Grimsley; B J Barnett

doi:10.1016/0003-2697(87)90186-2

Purification of ribonuclease T1

Anal Biochem. 1987 Dec;167(2):418-22. doi: 10.1016/0003-2697(87)90186-2.

Authors

C N Pace¹, G R Grimsley, B J Barnett

Affiliation

¹ Department of Biochemistry, Texas A&M University, College Station 77843.

PMID: 3126677
DOI: 10.1016/0003-2697(87)90186-2

Abstract

An improved method for purifying ribonuclease T1 from Aspergillus oryzae is described. The method uses gradient elution from DEAE-cellulose and sulfopropyl-Sephadex columns followed by gel filtration on Sephadex G-50 to give almost 100 mg (50% yield) of ribonuclease T1 from 100 g of starting material in less than 5 days.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Aspergillus oryzae / enzymology
Chromatography, DEAE-Cellulose
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Endoribonucleases / isolation & purification*
Ribonuclease T1 / isolation & purification*
Spectrophotometry

Substances

Endoribonucleases
Ribonuclease T1

Grants and funding

GM 37039/GM/NIGMS NIH HHS/United States