Host mitochondria influence gut microbiome diversity: A role for ROS

Sci Signal. 2019 Jul 2;12(588):eaaw3159. doi: 10.1126/scisignal.aaw3159.


Changes in the gut microbiota and the mitochondrial genome are both linked with the development of disease. To investigate why, we examined the gut microbiota of mice harboring various mutations in genes that alter mitochondrial function. These studies revealed that mitochondrial genetic variations altered the composition of the gut microbiota community. In cross-fostering studies, we found that although the initial microbiota community of newborn mice was that obtained from the nursing mother, the microbiota community progressed toward that characteristic of the microbiome of unfostered pups of the same genotype within 2 months. Analysis of the mitochondrial DNA variants associated with altered gut microbiota suggested that microbiome species diversity correlated with host reactive oxygen species (ROS) production. To determine whether the abundance of ROS could alter the gut microbiota, mice were aged, treated with N-acetylcysteine, or engineered to express the ROS scavenger catalase specifically within the mitochondria. All three conditions altered the microbiota from that initially established. Thus, these data suggest that the mitochondrial genotype modulates both ROS production and the species diversity of the gut microbiome, implying that the connection between the gut microbiome and common disease phenotypes might be due to underlying changes in mitochondrial function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Bacteria / classification
  • Bacteria / genetics
  • Catalase / genetics
  • Catalase / metabolism
  • DNA, Mitochondrial / genetics*
  • Gastrointestinal Microbiome / genetics*
  • Genetic Variation*
  • Genotype
  • Host Microbial Interactions / genetics
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Inbred NZB
  • Mitochondria / genetics*
  • Mitochondria / metabolism
  • NADH Dehydrogenase / genetics
  • NADH Dehydrogenase / metabolism
  • Phenotype
  • Reactive Oxygen Species / metabolism


  • DNA, Mitochondrial
  • Reactive Oxygen Species
  • Catalase
  • NADH Dehydrogenase
  • NADH dehydrogenase subunit 6, mouse