High-speed video recordings of escape responses in freely behaving crayfish revealed precisely coordinated movements of conspicuous head appendages, the antennal scales, during tail-flips that are produced by giant interneurons. For tail-flips that are generated by the medial giants (MG) in response to frontal attacks, the scales started to extend immediately after stimulation and extension was completed before the animal began to propel backwards. For tail-flips that are elicited by caudal stimuli and controlled by the lateral giants (LG), scale extensions began with significant delay after the tail-flip movement was initiated, and full extension of the scales coincided with full flexion of the tail. When we used implanted electrodes and stimulated the giant neurons directly, we observed the same patterns of scale extensions and corresponding timing. In addition, single action potentials of MG and LG neurons evoked with intracellular current injections in minimally restrained preparations were sufficient to activate scale extensions with similar delays as seen in freely behaving animals. Our results suggest that the giant interneurons, which have been assumed to be part of hardwired reflex circuits that lead to caudal motor outputs and stereotyped behavior, are also responsible for activating a pair of antennal scales with high temporal precision.
Keywords: Antennal scales; Crayfish; Giant interneurons; Sensory-motor integration; Tail-flip.