Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol

Methods Mol Biol. 2019;2013:83-90. doi: 10.1007/978-1-4939-9550-9_6.


The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.

Keywords: Antibodies; Antigens; ELISA; Optical density; Serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / analysis
  • Antibodies / immunology
  • Antigens / analysis
  • Antigens / immunology
  • Antimalarials / therapeutic use
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Malaria / diagnosis
  • Malaria / immunology


  • Antibodies
  • Antigens
  • Antimalarials