Alteration of MCP-1 and MMP-9 in Aqueous Humor Is Associated with Secondary Glaucoma in Fuchs Uveitis Syndrome

Ocul Immunol Inflamm. 2020 May 18;28(4):688-698. doi: 10.1080/09273948.2019.1609049. Epub 2019 Jul 3.


Purpose: To analyze changes in concentrations of pro- or anti-inflammatory cytokines, chemokines, or metalloproteinases (MMP) in the aqueous humor (AH) in Fuchs uveitis syndrome (FUS) patients with (FUSwG) or without (FUSwoG) secondary glaucoma.

Methods: AH samples were collected from 43 eyes of Caucasian subjects (FUSwoG: n = 11; FUSwG: n = 8; control eyes: n = 24). Concentrations of IL-8, MCP-1, MMP-1,-2,-3,-9, SAA, TGFß-1,-2,-3, and TNF-α were measured by multiplex bead assay analysis.

Results: Compared with the control group, levels of IL-8, MCP-1, MMP-3, and MMP-9 in the AH were significantly increased in FUSwG and FUSwoG patients. In contrast to FUSwoG patients, MCP-1 and MMP-9 level were lower in FUSwG, while expression of MMP-2, MMP-3, and TGFβ-1 was increased.

Conclusion: In our experiments, glaucoma in FUS patients was associated with low levels of MCP-1 and MMP-9 in the AH, while expression of MMP-2, MMP-3, and TGFβ-1 increased. The alteration in these molecular patterns may contribute to the development of glaucoma in FUS.

Keywords: Aqueous humor; Fuchs uveitis syndrome; intraocular pressure; multiplex bead assay analysis; secondary glaucoma.

MeSH terms

  • Adult
  • Aged
  • Aqueous Humor / metabolism*
  • Biomarkers / metabolism
  • Chemokine CCL2 / metabolism*
  • Cytokines / metabolism
  • Female
  • Glaucoma / etiology
  • Glaucoma / metabolism*
  • Humans
  • Male
  • Matrix Metalloproteinase 9 / metabolism*
  • Middle Aged
  • Syndrome
  • Uveitis / complications*
  • Uveitis / diagnosis
  • Uveitis / metabolism


  • Biomarkers
  • CCL2 protein, human
  • Chemokine CCL2
  • Cytokines
  • Matrix Metalloproteinase 9