Bacteria With Natural Chemotaxis Towards Methanol Revealed by Chemotaxis Fishing Technique

Biosci Biotechnol Biochem. 2019 Nov;83(11):2163-2171. doi: 10.1080/09168451.2019.1637715. Epub 2019 Jul 4.

Abstract

Motile bacteria often exhibit chemotaxis toward favorable compounds. However, the diversity of bacteria that are attracted to a given substance is largely unknown. This study aimed to reveal the diversity of bacteria with natural chemotaxis towards methanol. We tried to enrich environmental chemotactic bacteria using a glass capillary that is half-filled with methanol solidified with agarose as a trap ("chemotaxis fishing"). The pilot experiment using methanol-chemotactic Methylobacterium aquaticum strain 22A enriched the cells by 46-fold. The method was then applied to bacterial suspensions from paddy water and plants. Depending on the isolation sources and the methods of motility induction, methylotrophic bacteria were enriched 1.2-330-fold. The fished isolates belong to 32 species in 18 genera, mainly containing Acinetobacter, Methylobacterium and Pseudomonas species. Our chemotaxis fishing unveiled a part of diversity of the bacteria with natural chemotaxis towards methanol.

Keywords: Chemotaxis; Methylobacterium; methanol; plant-associated bacteria; whole-cell MALDI-TOF/MS.

MeSH terms

  • Bacteria / cytology*
  • Bacteria / drug effects*
  • Chemotaxis / drug effects*
  • Methanol / pharmacology*
  • Microbiological Techniques / methods*
  • Plants / microbiology

Substances

  • Methanol