The mitochondrial NAD + transporter (NDT1) plays important roles in cellular NAD + homeostasis in Arabidopsis thaliana

Plant J. 2019 Nov;100(3):487-504. doi: 10.1111/tpj.14452. Epub 2019 Aug 9.

Abstract

Nicotinamide adenine dinucleotide (NAD+ ) is an essential coenzyme required for all living organisms. In eukaryotic cells, the final step of NAD+ biosynthesis is exclusively cytosolic. Hence, NAD+ must be imported into organelles to support their metabolic functions. Three NAD+ transporters belonging to the mitochondrial carrier family (MCF) have been biochemically characterized in plants. AtNDT1 (At2g47490), focus of the current study, AtNDT2 (At1g25380), targeted to the inner mitochondrial membrane, and AtPXN (At2g39970), located in the peroxisomal membrane. Although AtNDT1 was presumed to reside in the chloroplast membrane, subcellular localization experiments with green fluorescent protein (GFP) fusions revealed that AtNDT1 locates exclusively in the mitochondrial membrane in stably transformed Arabidopsis plants. To understand the biological function of AtNDT1 in Arabidopsis, three transgenic lines containing an antisense construct of AtNDT1 under the control of the 35S promoter alongside a T-DNA insertional line were evaluated. Plants with reduced AtNDT1 expression displayed lower pollen viability, silique length, and higher rate of seed abortion. Furthermore, these plants also exhibited an increased leaf number and leaf area concomitant with higher photosynthetic rates and higher levels of sucrose and starch. Therefore, lower expression of AtNDT1 was associated with enhanced vegetative growth but severe impairment of the reproductive stage. These results are discussed in the context of the mitochondrial localization of AtNDT1 and its important role in the cellular NAD+ homeostasis for both metabolic and developmental processes in plants.

Keywords: Arabidopsis thaliana; nicotinamide adenine dinucleotide; pollen viability; starch metabolism; transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiporters / genetics
  • Antiporters / metabolism*
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / physiology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Biological Transport
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Chloroplasts / metabolism
  • Cytosol / metabolism
  • Gene Expression Regulation, Plant*
  • Green Fluorescent Proteins
  • Homeostasis
  • Mitochondria / metabolism
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Mutagenesis, Insertional
  • NAD / metabolism*
  • Nucleotide Transport Proteins
  • Peroxisomes / metabolism
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plant Leaves / physiology
  • Pollen / genetics
  • Pollen / growth & development
  • Pollen / physiology
  • Starch / metabolism

Substances

  • AT2G47490 protein, Arabidopsis
  • Antiporters
  • Arabidopsis Proteins
  • Carrier Proteins
  • Mitochondrial Proteins
  • Nucleotide Transport Proteins
  • NAD
  • Green Fluorescent Proteins
  • Starch