Remediation of PAHs contaminated soil using a sequence of soil washing with biosurfactant produced by Pseudomonas aeruginosa strain PF2 and electrokinetic oxidation of desorbed solution, effect of electrode modification with Fe3O4 nanoparticles

Sudabeh Pourfadakari; Mehdi Ahmadi; Neematollah Jaafarzadeh; Afshin Takdastan; Abd Alkazem Neisi; Shokouh Ghafari; Sahand Jorfi

doi:10.1016/j.jhazmat.2019.120839

Remediation of PAHs contaminated soil using a sequence of soil washing with biosurfactant produced by Pseudomonas aeruginosa strain PF2 and electrokinetic oxidation of desorbed solution, effect of electrode modification with Fe₃O₄ nanoparticles

J Hazard Mater. 2019 Nov 5:379:120839. doi: 10.1016/j.jhazmat.2019.120839. Epub 2019 Jun 27.

Authors

Sudabeh Pourfadakari¹, Mehdi Ahmadi², Neematollah Jaafarzadeh², Afshin Takdastan², Abd Alkazem Neisi², Shokouh Ghafari³, Sahand Jorfi⁴

Affiliations

¹ Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
² Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
³ Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
⁴ Department of Environmental Health Engineering, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: sahand369@yahoo.com.

PMID: 31279313
DOI: 10.1016/j.jhazmat.2019.120839

Abstract

This work aimed to investigate the performance of biosurfactant, produced by a halotolerant bacterial strain, Pseudomonas aeruginosa PF2, for desorption of PAHs from soil, followed by electrokinetic oxidation of the desorbed solution using Magnetite Nanoparticles Modified Graphite (MNMG). Pyrene (PYR), anthracene (ANT) and phenanthrene (PHE) were used as contamination model. Produced and extracted biosurfactant was characterized as rhamnolipid with Critical Micelle Concentration (CMC) of 60 mg/L and emulsification index (E₂₄) value of 60.2% for n-hexadecane, 58.4% for n-heptane and 55.6% for n-Hexane, respectively. Results of LC-MS/MS analysis indicated the presence of seven major peaks at m/z of 677.5, 531.1, 649.3, 528.9, 475.1, 359 and 503.2, which corresponded to the deprotonated molecules of RhaRhaC₁₂C_10, RhaC₁₂C_10, RhaRhaC₁₀C₁₀, RhaC_12:1C_10, RhaC₈C_10, Rha-C₁₂:₂ and RhaC₁₀C_10, respectively. The maximum desorption of PAHs was derived at pH value of 6, CMC of 3 and contact time of 24 h. Modification of graphite electrode enhanced the PAH degradation significantly. In electrokinetic oxidation of desorbed solution, the best results were observed at pH value of 5, contact time of 6 h, voltage of 3 V and electrolyte concentration of 25 mg/L, with the average removal efficiency of higher than 99% for all studied PAHs.

Keywords: Biosurfactant; Electrokinetic oxidation; Fe(3)O(4) nanoparticles; PAHs; Soil washing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrochemical Techniques / methods*
Electrodes
Environmental Restoration and Remediation / methods*
Magnetite Nanoparticles / chemistry*
Oxidation-Reduction
Polycyclic Aromatic Hydrocarbons / analysis*
Pseudomonas aeruginosa / metabolism*
Soil Pollutants / analysis*
Surface-Active Agents / chemistry*
Surface-Active Agents / isolation & purification

Substances

Magnetite Nanoparticles
Polycyclic Aromatic Hydrocarbons
Soil Pollutants
Surface-Active Agents