Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment

Curr Opin Struct Biol. 2019 Oct;58:259-268. doi: 10.1016/ Epub 2019 Jul 3.


Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cryoelectron Microscopy / methods*
  • Membrane Proteins / chemistry*
  • Membranes, Artificial*


  • Membrane Proteins
  • Membranes, Artificial